Lab Report on TLC analisys of Analgestic Drugs Essay

In this experiment, thin-layer chromatography (TLC) was use to charm the composition of various nonprescription(prenominal) (OTC) analgesics Anacin, Bufferin, Excedrin, and Tylenol. The TLC plates were first viewed under ultraviolet (UV) light and then(prenominal) hardened with single vapor in order to visualize the spotting. Experiment purposeIniti tout ensembley, sixteen capillary micropipets were created in order to spot the TLC plates. deuce TLC plates were then obtained and smeared with pencil for spotting. A line was move 1 cm from the bottom of each plate, and five small, evenly set-apart marks were made along those lines (see mannequin 1). Each mark indicated where a substance would be descry.All compounds used were in solutions of 1g of each fade away in 20 ml of a 5050 multifariousness of methylene chloride and ethanol. The first plate made was the reference plate. Capillary micropipets were used to spot the first four marks with acetaminophen, aspirin, caffein e, and salicylamide (in that order). (See figures 2-5 for chemical structures.)The last mark was spotted with a reference solution of all four chemicals. The game plate made was the sample plate. The first four marks were spotted with Anacin, Bufferin, Excedrin, and Tylenol. The fifth mark was spotted with a reference solution of all four drugs. Figure 1. Prepared TLC platesFigure 2. AcetaminophenFigure 3. AspirinFigure 4. CaffeineFigure 5. SalicylamideA development container was created with a wide-mouthed screwcap jar. It was filled with the development resoluteness, which was .5% glacial acetic acid in ethyl acetate, so that the solvent was approximately .5 cm deep.The first TLC plate was then carefully dictated into the development container. Great care was taken to ensure that the plate went in evenly so that the solvent could rise evenly up the plate. at once the solvent wait had reached approximately 1cm from the top of the plate, the plate was removed, the solvent front was marked with a pencil, and the plate was allowed to dry.The second plate was then rigid in the development chamber in the same manner as the first. Once the solvent front reached approximately 1cm from the top of the plate, the plate was removed, the solvent front was marked with a pencil, and the plate was allowed to dry. Each plate was then viewed under the UV light.Any spots that were seen were lightly circled with a pencil, and their food colouring was noted. The orders of elution (Rf values) were calculated by dividing the distance from the baseline to the center of the spot by the distance from the baseline to the solvent front. After all observations and calculations were made, the plates were placed in a jar containing iodine.The jar was warmed with hands so that the iodine vaporized. The plates were then removed from the jar and observed. The reference and sample plates were then compared to determine which compounds the drugs on the sample plate contained. Data